Product: APC anti-mouse Ki-67

Catalog No.: 652405

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

Anti-mouse Ki-67 APC for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Good option to stain Ki-67.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse splenocytes
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti surface antibodies. Incubate 15min at 4°C in the dark. Wash. Fix and permeabilize the cells. Wash. Use anti-ki67 APC at (1/100) in perm/wash buffer. Incubate 30min in the dark. Wash with Perm/wash buffer. Resuspend in PBS/BSA and transfer to FACS tubes to read.
Results Summary:	CD138hi cells (red) containing plasmablast and plasma cells contain higher frequency of proliferating cells (Ki67+), than B cells (blue).
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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