Product: Brilliant Violet 421™ anti-mouse NK-1.1 |
Catalog No.: 108731 |
Stehle Christina, PhD student DRFZ Berlin |
NK1.1 on Mouse Liver Cells (Brilliant Violet 421™) | |||
Overall: | Product Quality: | Ease of Use: | |
C57BL/6 mouse liver T-bet+ cells express NK1.1. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | C57BL/6 mouse liver cells |
Brief Protocol: | FACS staining: -5 min Fc block incubation -addition of antibody cocktail mix, staining for 30 min at 4°C in the dark -wash in PBS/BSA, centrifuge at 350 g 6 min -intracellular fixation and permeabilization -intracellular staining with anti-T-bet -wash in buffer, centrifuge at 350 g 6 min -resuspend in PBS/BSA |
Results Summary: | Hepatic T-bet+ cells express NK1.1 on their surface. |
Additional Notes: |
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