Product: Brilliant Violet 421™ anti-mouse CD8a

Catalog No.: 100737

Mikhail Binnewies, Graduate Student UCSF

Anti-CD8a Brilliant Violet 421™ for Identifying Resident DC Subsets in the LN
Overall:	Product Quality:	Ease of Use:
Anti-CD8a Brilliant Violet 421™ staining works well following collagenase I, IV and DNAse digestion in the lymph node.

Experimental Design

Application:	Flow cytometry
Cells used:	Enzymatically digested lymph node cells
Brief Protocol:	Inguinal lymph nodes were pierced and dissociated in 100 U/ml collagenase I, 400 U/ml collagenase IV and 200 ug/ml DNAse I for 30 minutes at 37C. Digested cells were filtered, washed with DPBS and stained with fixable Zombie NIR™. Cells were washed with PBS/2% FCS/2mM EDTA and stained with a panel to identify T cells, B cells, and DCs, and then anti-CD11b Brilliant Violet 605™ (1:1000) and anti-CD8a Brilliant Violet BV421™ (1:400). Plot shown below is after gating out B and T cells and enriching on resident DCs.
Results Summary:	Staining of anti-CD8a Brilliant Violet 421™ works very well following enzymatic digestion.
Additional Notes:	I would not recommend lowering the concentration of the antibody below 1:400.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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