Product: ELISA MAX™ Deluxe Set Human TNF-a

Catalog No.: 430205

Ohad Tarcic, Dr. Weizmann

TNF-α Human (ELISA MAX™ Deluxe)
Overall:	Product Quality:	Ease of Use:
Good TNF- alpha detection kit.

Experimental Design

Application:	ELISA
Cells used:	Human mammary cells
Brief Protocol:	1. Coat the plates with capture antibody. 2. Block with blocking solution for 2 hours and then wash with PBST. 3. Add samples and standards to each well in triplicates for 2-4 hours in RT. 4. Add detection antibody for 1 hour and then wash with PBST. 5. Add abiding HRP for 30 minutes and then wash with PBST. 6. Add TMB and then read OD at 450nm.
Results Summary:	TNF alpha detection.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews