Product: Pacific Blue™ anti-mouse/human CD11b

Catalog No.: 101223

Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute

CD11b Pacific Blue™ for Flow Cytometry
Overall:	Product Quality:	Ease of Use:
Very good and bright antibody.

Experimental Design

Application:	Flow cytometry
Cells used:	Mouse peritoneal cavity
Brief Protocol:	Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (24.62) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl of antibody staining solution containing CD11b Pacific Blue™(1:1600). Incubate 15min at 4°C in the dark. Wash with PBS/BSA, spin down resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells.
Results Summary:	Macrophages are higher expressors of CD11b, while B cells (B-1 cells) that are enriched in the peritoneal cavity are intermediate expressors of CD11b.
Additional Notes:	This antibody is very good to sort macrophages and B cells from the peritoneal cavity.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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