Product: ELISA MAX™ Standard Set Mouse IL-6

Catalog No.: 431302

Midic Ivana, PhD University of Montenegro

Mouse IL-6 ELISA MAX™ Standard
Overall:	Product Quality:	Ease of Use:
Confirming the inflammatory cytokines produced from macrophages under experimental conditions.

Experimental Design

Application:	ELISA
Cells used:	Macrophages
Brief Protocol:	1) Dilute capture antibody to the coating buffer as prescribed in the sheets and add it to the plate. 2) After overnight incubation wash + add block the plate. 3) After 1 hour wash + add standards and samples. 4) After 2 hours wash + add detection antibody. 5) After 1 hour wash + add the enzyme avidin HRP. 6) After 30 minutes wash + add TMB solution. 7) After 10-20 minutes stop the reaction using an acid. 8) Read the plate.
Results Summary:	Confirming the inflammatory cytokines produced from macrophages under experimental conditions.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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