Product: Brilliant Violet 605™ anti-mouse/human CD11b |
Catalog No.: 101257 |
Nathan Jacobs, PhD Student Emory University |
Great CD11b/CD11c Labeling for Innate Cell Populations | |||
Overall: | Product Quality: | Ease of Use: | |
BioLegend's antibodies make CD11b vs. CD11c stratification, the first step in innate cell analysis a breeze. |
Experimental Design |
Application: | Splenocyte staining during malaria |
Cells used: | Splenocytes |
Brief Protocol: | Spleens were removed from mice on days 5, 7, 10, and 15 of infection with Plasmodium chabaudi. Spleens were mashed through a 100 um filter to release cells, RBC lysed, counted, and 2*10^6 cells were Fc blocked (15 min @ 4ºC), then stained (30 min @ 4º C). Samples were analyzed on a BD LSR-II. |
Results Summary: | This antibody helps us identify unique combinations of CD11b/CD11c expression on populations of interest. |
Additional Notes: |
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