Product: ELISA MAX™ Standard Set Mouse IL-6

Catalog No.: 431301

Goutham Pattabiraman, Postdoctoral Fellow University of Connecticut Health Center

Mouse IL-6 ELISA
Overall:	Product Quality:	Ease of Use:
ELISA for cell culture supernatants from BMDMs

Experimental Design

Application:	ELISA
Cells used:	Bone Marrow derived macropghages
Brief Protocol:	Dilute capture antibody to the coating buffer as prescribed in the sheets and add it to the plate. After overnight incubation, wash, add block the plate. After 1 hour wash, add standards and samples. After 2 hours, wash, add detection antibody. After 1 hour, wash, add the enzyme avidin HRP. After 30 minutes, wash, add TMB solution, and after 10-20 minutes, add stop the reaction using an acid. Read the plate.
Results Summary:	Good range of detection
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

See all Reviews