Non-specific binding is only a staining artifact when tandem-labeled antibodies are applied to live cells. Our data indicates that short term exposure (30 minutes) of the True-Stain Monocyte Blocker™ itself does not have any stimulatory effect sufficient to induce cytokine production in PBMCs. The data below demonstrates the lack of IL-4, IFN-γ, and TNFα production under these conditions. In addition, no substantial effect on cell viability or cell activation could be detected in PMA/Ionomycin activated or unstimulated CD4+, CD8+ or CD14+ cell populations (data not shown).
However, longer incubation times or alternative stimulation conditions, such as TLR or other signaling pathways, would need to be further defined in order to fully assess potential unwanted stimulatory effects from the Blocker solution.