TotalSeq™-D0817 anti-human CD109 Antibody

Pricing & Availability
Clone
W7C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gov platelet alloantigen
Isotype
Mouse IgG1, κ
Barcode Sequence
CACTTAACTCTGGGT
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Product Citations
publications
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323313 10 µg £222.00
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Description

The W7C5 monoclonal antibody recognizes human CD109 also known as the Gov platelet alloantigen. CD109 is a member of the alpha2-macroglobulin/complement gene family that is a GPI-linked cell surface antigen with a predicted molecular weight approximately 162 kD. CD109 contains a thioester motif characteristic of alpha-2-macroglobulin that undergoes autolytic cleavage under denaturing conditions. CD109 expression has been reported on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells. It is also widely expressed in the brain, uterus, heart, lung, and trachea of adults. The function of CD109 is largely unknown although it has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

 


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Additional Product Notes

TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.

Application References

(PubMed link indicates BioLegend citation)
  1. Giesert C, et al. In:Hematopoietic Stem Cells 2002:Genetics and function.
  2. Orlic D, et al. 2003. Ann. New York Acad. Sci. 996:227.
  3. Vogel W, et al. 2002. Haematologica 88:126.
RRID
AB_2894679 (BioLegend Cat. No. 323313)

Antigen Details

Structure
Member of the alpha2-macroglobulin/complement gene family. GPI-linked cell surface antigen, predicted molecular weight approximately 162 kD.
Distribution

Expressed on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells.

Function
Unknown; has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura
Cell Type
Hematopoietic stem and progenitors, Leukemia, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lin M, et al. 2002. Blood 99:1683.
  2. Schuh AC, et al. 2002. Blood 99:1692.
  3. Solomon KR, et al. 2004. Gene 327:171.
Gene ID
135228 View all products for this Gene ID
UniProt
View information about CD109 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/14/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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