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Purified anti-Merlin (NF2) Antibody

Pricing & Availability
Clone
W19231A (See other available formats)
Regulatory Status
RUO
Other Names
Neurofibromin 2, NF2, Merlin, Schwannomin, SCH, Neurofibromin 2 (Bilateral Acoustic Neuroma), Moesin-Ezrin-Radixin-Like Protein, Moesin-Ezrin-Radixin Like, Schwannomerlin, BANF, ACN
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19231A_PURE_Merlin_Antibody_1_040721.png
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-Merlin antibody (clone W19231A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19231A_PURE_Merlin_Antibody_1_040721.png
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-Merlin antibody (clone W19231A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19231A_PURE_Merlin_Antibody_2_040721.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) or purified anti-Merlin antibody (clone W19231A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a control antibody raised against a separate epitope of Merlin. Lane M: Molecular weight marker.
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945201 25 µg £81
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945202 100 µg £201
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Description

Moesin-ezrin-radixin-like protein (Merlin) is a tumor suppressor protein encoded by the Neurofibromin 2 (NF2) gene. It functions as a scaffold linking the plasma membrane and the cytoskeleton, and plays a crucial role in regulating cell polarization and morphology. Merlin functions as a major upstream activator of the Hippo signaling pathway, where it promotes Lats1/2 degradation, leading to increased nuclear localization of the YAP/TAZ transcription factors. Mutations and deletions within the NF2 gene cause neurofibromatosis type 2, a tumorigenic disease of the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to human Merlin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100, and methanol-fixed and permeabilized HeLa cells. None of the methods were compatible with Merlin staining.

This clone is not predicted to cross-react with other Merlin homologs due to poor sequence conservation between the immunizing peptide and the corresponding region in each homolog.

RRID
AB_2892510 (BioLegend Cat. No. 945201)
AB_2892510 (BioLegend Cat. No. 945202)

Antigen Details

Structure
Merlin is a 595 amino acid protein with a predicted molecular weight of 70 kD.
Distribution

Ubiquitously expressed/Cytosol

Function
Tumor suppression, apoptosis
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References
  1. Li W, et al. 2010. Cell. 140:477-490
  2. Petrilli A, et al. 2016. Oncogene. 35:537-548.
  3. White S, et al. 2019. Dev Cell. 49:425-443.E9.
Gene ID
4771 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Merlin
Specificity Alt (DOES NOT SHOW ON TDS):
Merlin
App Abbreviation (DOES NOT SHOW ON TDS):
WB,IP
UniProt
View information about Merlin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/07/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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