Purified anti-KEAP1 Antibody

Pricing & Availability
Clone
W19335C (See other available formats)
Regulatory Status
RUO
Other Names
Kelch Like ECH Associated Protein 1, Cytosolic Inhibitor Of Nrf2, KLHL19
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Whole cell extracts (15 µg protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-KEAP1 antibody (clone W19335C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of increasing predicted KEAP1 expression (source: Human Protein Atlas).
  • Whole cell extracts (15 µg protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-KEAP1 antibody (clone W19335C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of increasing predicted KEAP1 expression (source: Human Protein Atlas).
Cat # Size Price Quantity Check Availability Save
944601 25 µg £68.00
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944602 100 µg £171.00
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Description

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. Active NRF2 binds to antioxidant response elements (AREs) located in the regulatory promoter of target genes and induces expression of these genes. NRF2 is degraded through ubiquitination by an E3 ligase complex that contains KEAP1 as an adaptor protein. Under normal conditions, KEAP1 directly binds and sequesters NRF2 in the cytosol and promotes its ubiquitinatation and proteasomal degradation. In response to elevated oxidative stress, reactive cysteine residues on KEAP1 are modified, resulting in a conformational change that causes the dissociation of NRF2 from KEAP1, and an accumulation of NRF2 in the nucleus. In a clinical setting, NRF2 is frequently activated in many types of cancers, driving metabolic adaptation and survival in ROS-rich tumor microenvironments. In KRAS-mutant cancers, loss of KEAP1 promotes dependence on glutaminolysis through upregulation of NRF2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human KEAP1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892508 (BioLegend Cat. No. 944601)
AB_2892508 (BioLegend Cat. No. 944602)

Antigen Details

Structure
KEAP1 is a 624 amino acid protein with a predicted molecular weight of 70 kD.
Distribution

Nucleus and cytoplasm/Ubiquitously expressed

Function
E3 ligase, NRF2 regulation
Interaction
NRF2
Biology Area
Cell Biology, Ubiquitin/Protein Degradation
Molecular Family
Protein Modifiers
Antigen References
  1. Romero R, et al. 2017. Nat Med. 23:1362.
  2. Shoemaker A. 2017. Sci Trans Med. 9:420.
  3. M. da Costa R, et al. 2019. Front In Pharm. 10:3389.
  4. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155.
  5. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.
Gene ID
9817 View all products for this Gene ID
UniProt
View information about KEAP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/06/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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