*Antigen presenting cell subsets (and other cells here) can express different markers and be challenging to analyze. To learn more about your specific cell type, check out our Explore and Learn section.
‡This marker’s expression is variable, but is used to further analyze subclasses of this cell type.
Examining CD45 expression and forward and side scatter profiles can help you identify your population of interest. The image below was edited from Im, M. et al.
Human peripheral blood leukocytes (A) after staining with anti-CD45 showing discrete granulocyte (P1), monocyte (P2), and lymphocyte (P3) populations according to the level of CD45 expression. (B) FSC and SSC on the scattergrams.
- Sunderkötter, C. et al. 2004. J. Immunol. 172:4410. Pubmed (Mouse Monocytes)
- Murray, P.J. and Wynn, T.A. 2011. Nat. Rev. Immunol. 11:723. Pubmed (Human Macrophages)
- Zhang, X. et al. 2008. Curr. Protoc. Immunol. Chapter 14:Unit 14.1 Pubmed (Mouse Macrophages)
- Moore, A.J. and Anderson, M.K. 2013. Adv. Hematol. 2013:949513. Pubmed (Human and Mouse Dendritic Cells)
- Lee, J.J. et al. 2012. J. Allergy Clin. Immunol. 130:572. Pubmed (Human and Mouse Eosinophils)
- Macey, M.G. Flow Cytometry: Principles and Applications. Totowa: Humana Press Inc. 2007. Digital. Link (Human Neutrophils)
- Kim, H.J. et al. 2010. PLoS One. 5:e11439. Pubmed (Mouse Neutrophils)
- Maecker, H.T. et al. 2012. Nat. Rev. Immunol. 12:191. Pubmed (General Human Markers)
- Lai, L. et al. 1998. J. Immunol. 160:3861. Pubmed (General Mouse Markers)