To complement our collection of TotalSeq™ antibodies, we now offer antibody-oligonucleotide conjugates to allow for sample multiplexing and robust multiplet discrimination during single cell analysis. These cell Hashtag reagents are a pool of antibodies conjugated to a unique barcode sequence and are designed to cover as many cells as possible in both human and mouse samples. For human samples, the Hashtag reagents are comprised of antibodies detecting CD298 and β2 microglobulin. For mouse samples, the Hashtag reagents contain antibodies against CD45 and H-2 MHC class I. Because the cell hashing reagents use a different amplification handle than our TotalSeq™ reagents, immunophenotyping, Hashtags, and mRNA libraries can be independently amplified and pooled at desired ratios for sequencing.

Left: Hashtag dataset resolving 4 PBMC donors multiplexed into one single cell run. Multiplets (grey) form distinct smaller populations that can be removed from analysis. Right: Transcriptome-based clustering reveals distinct immune cell populations interspersed across all four samples. Cells are colored based on their hashtag classification.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer’s intended uses of the BioLegend TotalSeq™ products.  For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer’s sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

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