HUVEC cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human/mouse CD44 (clone IM7) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40x objective.
Human peripheral mononuclear cell and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA) and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective.
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellular stained with 2.5 µg/ml of Ki-67 (clone Ki-67) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40x objective.
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellular stained with 5 µg/ml of Tubulin-alpha (clone 10D8) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40x objective.