Human/mouse CD44, clone IM7

HUVEC cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml of anti-human/mouse CD44 (clone IM7) Alexa Fluor® 594 (red) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40x objective.