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Purified anti-Maltose Binding Protein (MBP) Antibody (Previously Covance catalog# MRT-131P)

Pricing & Availability
Clone
YM-2 (See other available formats)
Regulatory Status
RUO
Other Names
ECK4026, JW3994, Mal E, MalE, malJ, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, MMBP, Periplasmic maltose binding protein
Previously
Covance Catalog# MRT-131P
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YM-2_PURE_Maltose_Binding_Protein_Antibody_WB_122017
Total lysates (15 µg protein) from BL21 (E. coli) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified anti-Maltose Binding Protein antibody, clone YM-2. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution).
  • YM-2_PURE_Maltose_Binding_Protein_Antibody_WB_122017
    Total lysates (15 µg protein) from BL21 (E. coli) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified anti-Maltose Binding Protein antibody, clone YM-2. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution).
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906901 200 µL 212€
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Description

The maltose-binding protein (MBP) makes up a part of the maltose/maltodextrin system in E. coli that is responsible for the uptake and catabolism of maltodextrins. It has a predicted molecular weight of 42.5 kDa and is used to increase the solubility of E. coli expressed recombinant proteins, or as an affinity tag for the purification of recombinant proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
YM-2 was raised against a recombinant MBP (maltose-binding protein) from E. coli.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000

Application Notes

This antibody is effective in immunoblotting and ELISA.
 

Application References

(PubMed link indicates BioLegend citation)
  1. Tian W, et al. 2004. Cancer Sci. 95:491.
Product Citations
  1. Tsourouktsoglou TD, et al. 2020. Cell Reports. 31(5):107602. PubMed
  2. Ma X, et al. 2020. Nature. 581:199. PubMed
RRID
AB_2565070 (BioLegend Cat. No. 906901)

Related FAQs

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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