Enzymes and Regulators

Enzymes act as biological catalysts to regulate chemical reactions and are highly specific to the substrates with which they react. Through these reactions, enzymes and their regulators (such as inhibitors) modulate cell fate, localization, and activity of many proteins. Additionally, enzymes can regulate protein-protein interactions, create new bioactive molecules, and contribute to signal transduction. Through these actions, enzymes influence cellular functions such as proliferation, differentiation, DNA replication, inflammation, and apoptosis.

 

 

Featured Data

Recombinant Human Elafin (carrier-free)

 

 

 

The activity of hElafin was measured by its ability to inhibit human neutrophil elastase. The potency of inhibition was measured by monitoring the cleavage of a fluorogenic substrate MeO-Suc-AAPV-AMC (100 μM) in the presence of human neutrophil elastase (100 ng/mL). The IC50 value of hElafin is less than 3.0 nM (34 ng/mL).

 

Recombinant Human MMP-10 (carrier-free)

 

 

 

The activity of human MMP-10 was measured with 10 μM of fluorogenic MMP substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, in the presence of 6.25, 12.5, 25, 50, 100 ng of activated hMMP-10. The activity of activated hMMP-10 is >200 pmole/min/μg.