Cytokines allow cells to communicate with one another and induce a wide range of activities. They can be pro- or anti-inflammatory, promote cell growth, or influence differentiation to various cell types. To mediate these functions, cytokines bind to their respective receptors on the cell surface, initiating signaling cascades for gene transcription.
Naïve CD4+ T cells can give rise to a number of T helper classes, depending on the recombinant proteins used to induce differentiation.
Recombinant Human TGF-β1(carrier-free)
Recombinant human TGF-β1 inhibits the proliferation of mouse HT-2 cell line induced by IL-4 in a dose-dependent manner. BioLegend's protein was compared side-by-side to the leading competitor's equivalent.
Th17 Polarization of Mouse CD4 Cells
Mouse CD4+ T cells were polarized with plate-bound anti-mouse CD3, soluble anti-mouse CD28, recombinant mouse IL-6, IL-23, and TGF-β, and anti-mouse IFN-γ for 4 days. After re-stimulation with PMA/ionomycin in the presence of BFA or monensin, the cells were harvested and surface stained with CD4 FITC, and intracellularly stained with IFN-γ APC and IL-17 PE.
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