- Clone
- M-A251 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV A053
- Other Names
- IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 FITC and anti-human CD25 (clone M-A251) Spark YG™ 581 (left) or CD4 FITC only (right). -
Human peripheral blood lymphocytes were stained with anti-human CD4 FITC and anti-human CD25 (clone M-A251) Spark YG™ 581 (left) or anti-human CD4 FITC only (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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356153 | 25 tests | 100€ | ||||
356154 | 100 tests | 212€ |
CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PHA-induced lymphocyte cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm. - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1 and spatial biology (IBEX)2,3.
The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde. -
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2890784 (BioLegend Cat. No. 356153)
AB_2890784 (BioLegend Cat. No. 356154)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
- Distribution
-
Activated T and B cells, monocytes/macrophages, Tregs
- Interaction
- Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
Other Formats
View All CD25 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC/Cyanine7 anti-human CD25
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Purified anti-human CD25
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PE anti-human CD25
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FITC anti-human CD25
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PE/Cyanine7 anti-human CD25
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APC anti-human CD25
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PerCP/Cyanine5.5 anti-human CD25
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Brilliant Violet 421™ anti-human CD25
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Alexa Fluor® 488 anti-human CD25
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Alexa Fluor® 700 anti-human CD25
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Brilliant Violet 510™ anti-human CD25
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PE/Dazzle™ 594 anti-human CD25
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Biotin anti-human CD25
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Alexa Fluor® 647 anti-human CD25
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Pacific Blue™ anti-human CD25
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PerCP anti-human CD25
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APC/Fire™ 750 anti-human CD25
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Brilliant Violet 711™ anti-human CD25
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Brilliant Violet 785™ anti-human CD25
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Brilliant Violet 605™ anti-human CD25
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KIRAVIA Blue 520™ anti-human CD25
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PE/Fire™ 700 anti-human CD25
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APC/Fire™ 810 anti-human CD25
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Spark NIR™ 685 anti-human CD25 Antibody
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Spark YG™ 581 anti-human CD25
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PE/Fire™ 640 anti-human CD25 Antibody
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PE anti-human CD25
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PerCP/Cyanine5.5 anti-human CD25
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APC/Fire™ 750 anti-human CD25
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PE/Cyanine7 anti-human CD25
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APC anti-human CD25
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PE/Cyanine5 anti-human CD25
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FITC anti-human CD25
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Spark Red™ 718 anti-human CD25
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GMP PE anti-human CD25
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