Spark YG™ 581 anti-human CD25 Antibody, CD25, M-A251

Pricing & Availability

Clone: M-A251 (See other available formats)
Regulatory Status: RUO
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ
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Product Citations: publications

PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 FITC and anti-human CD25 (clone M-A251) Spark YG™ 581 (left) or CD4 FITC only (right).

Compare all formats See Spark YG™ 581 spectral data

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356153

25 tests

100€

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356154

100 tests

212€

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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human PHA-induced lymphocyte cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections¹ and spatial biology (IBEX)^2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Application References (PubMed link indicates BioLegend citation)

Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2890784 (BioLegend Cat. No. 356153)
AB_2890784 (BioLegend Cat. No. 356154)

Antigen Details

Structure: Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution: Activated T and B cells, monocytes/macrophages, Tregs
Interaction: Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

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Other Formats

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Description	Clone	Applications
APC/Cyanine7 anti-human CD25	M-A251	FC
Purified anti-human CD25	M-A251	FC,IHC-F
PE anti-human CD25	M-A251	FC
FITC anti-human CD25	M-A251	FC
PE/Cyanine7 anti-human CD25	M-A251	FC
APC anti-human CD25	M-A251	FC
PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
Brilliant Violet 421™ anti-human CD25	M-A251	FC
Alexa Fluor® 488 anti-human CD25	M-A251	FC
Alexa Fluor® 700 anti-human CD25	M-A251	FC
Brilliant Violet 510™ anti-human CD25	M-A251	FC
PE/Dazzle™ 594 anti-human CD25	M-A251	FC
Biotin anti-human CD25	M-A251	FC
Alexa Fluor® 647 anti-human CD25	M-A251	FC,SB
Pacific Blue™ anti-human CD25	M-A251	FC
PerCP anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
Brilliant Violet 711™ anti-human CD25	M-A251	FC
Brilliant Violet 785™ anti-human CD25	M-A251	FC
Brilliant Violet 605™ anti-human CD25	M-A251	FC
KIRAVIA Blue 520™ anti-human CD25	M-A251	FC
PE/Fire™ 700 anti-human CD25	M-A251	FC
APC/Fire™ 810 anti-human CD25	M-A251	FC
Spark NIR™ 685 anti-human CD25 Antibody	M-A251	FC
Spark YG™ 581 anti-human CD25	M-A251	FC
PE/Fire™ 640 anti-human CD25 Antibody	M-A251	FC
PE anti-human CD25	M-A251	FC
PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
PE/Cyanine7 anti-human CD25	M-A251	FC
APC anti-human CD25	M-A251	FC
PE/Cyanine5 anti-human CD25	M-A251	FC
FITC anti-human CD25	M-A251	FC
Spark Red™ 718 anti-human CD25	M-A251	FC
GMP PE anti-human CD25	M-A251	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12.17.2020

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