Recombinant Rat IL-33 (carrier-free)

Pricing & Availability
Other Names
Interleukin-33, IL-33, Interleukin-1 Family, Member 11 (IL-1F11), Nuclear Factor From High Endothelial Venules (NF-HEV)
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Rat_IL-33_RECOM_CF_070617
Rat IL-33 induces the proliferation of D10.G4.1 mouse cells.
  • Rat_IL-33_RECOM_CF_070617
    Rat IL-33 induces the proliferation of D10.G4.1 mouse cells.
Cat # Size Price Quantity Avail. Save
766402 10 µg 140€
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766404 25 µg 192€
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766406 100 µg 484€
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766408 500 µg 1.760€
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Description

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1β. IL-33, IL-1β, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signaling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties. IL-33 plays a role in allergic disease, including allergic rhinitis and chronic rhinosinusitus and is elevated in humans and mouse models of atopic dermatitis.

Product Details
Technical data sheet

Product Details

Source
Rat IL-33, amino acid Ser109-Met264 (Accession# NP_001014188) was expressed in E. coli.
Molecular Mass
The 156 amino acid recombinant protein has a predicted molecular mass of approximately 17.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU/µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50= 0.05 - 0.25 ng/ml, as determined by the dose dependent stimulation of D10.G4.1 cell proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Higher levels of rat IL-33 mRNA are detected in lung, kidney, uterus, liver and thymus. Expressed in endothelial and epithelial cells, spleen fibroblastic reticular cells, rat glioma cell.

Function
IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-α and IL-1β are activators of IL-33 transcription in fibroblasts and keratinocytes.
Interaction
Th2 cells, group 2 innate lymphoid cells (ILC2s), mast cells, subsets of Tregs, inflammatory cells, including NK cells, Th1 cells, and CD8+ T cells.
Ligand/Receptor
IL-33 binds to the receptor IL-1RL1 (ST2) and the coreceptor IL-1RAcP (IL-1 receptor associated protein).
Bioactivity
Rat IL-33 induces the proliferation of D10.G4.1 cells.
Biology Area
Stem Cells, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schmitz J, et al. 2005. Immunity 23:479.
2. Barksby HE, et al. 2007. Clin. Exp. Immunol 149:217.
3. Arend WP, et al. 2008. Immunol. Rev 223:20.
4. Suzukawa M, et al. 2008. J. Immunol. 181:5981.
5. Moussio C, et al. 2008. PLoS One 3(10):e3331.
6. Fang KM, et al. 2014 Neuro Oncology 16:552.
7. Molofsky AB, et al. 2015 Immunity 42:1005. 

Gene ID
361749 View all products for this Gene ID
UniProt
View information about IL-33 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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