- Regulatory Status
- Other Names
- CD62 antigen-like family member P (CD62P), Granule membrane protein 140 (GMP-140), Leukocyte-endothelial cell adhesion molecule 3 (LECAM3), Platelet activation dependent granule-external membrane protein (PADGEM)
- Ave. Rating
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- Product Citations
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P-Selectin, an adhesion molecule, belongs to the selectin/LECAM family. P-Selectin consists of one Lectin domain and several EGF-like repeats, which are followed by a transmembrane region and a short cytoplasmic tail of 35 amino acids. P-Selectin is expressed in endothelial cells and megakaryocytes. In unstimulated cells, P-Selectin is synthesized and stored in the alpha-granules of platelets and the Weibel-Palade bodies of endothelial cells. Upon activation or exposure of stimuli, such as thrombin and histamine, P-Selectin is rapidly translocated from storage granule to the cell membrane within minutes and then is recycled back via endocytosis. Inflammatory stimuli, including TNF-α and endotoxins, can induce P-Selectin expression at the transcriptional and protein levels in mouse endothelial cells, but not in HUVEC. IL-4 and oncostatin M can induce P-Selectin expression in HUVEC cells. Surface P-Selectin binds to its ligand, PSGL-1, which is a glycoprotein that is expressed in neutrophils, monocytes, and dendritic cells. Its interaction initiates cell contact and selectin-mediated leukocyte rolling on the endothelial cells’ surfaces. In addition, some evidence has been shown that P-Selectin contributes to cancer metastasis. The interaction between tumor cells and platelets via P-Selectin facilitates adhesion of tumor cells in the vasculature.Product Details
- Mouse P-Selectin, amino acids (Trp42-Ala709) (Accession# BC1019158), with a linker (GGGGSR) and a C-terminal human IgG1 (Pro100-Lys330), was expressed in 293E cells.
- Molecular Mass
- The 905 amino acid recombinant protein has a predicted molecular mass of approximately 98.98 kD. The beta-Mercaptoethanol-reduced and non-reduced protein migrates at approximately 100 and 200 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Trp.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Immobilized mouse P-Selectin at 2 µg/mL (100 µL/well) induces 90-100% adhesion of U937 cells. The attached cell number was measured by the colorimetric MTS assay.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
- Product Citations
- Adhesion molecule.
Megakaryocytes (platelets) and endothelial cells.
- P-Selectin mediates leukocyte binding to endothelial cells during inflammatory responses.
- P-Selectin glycoprotein ligand-1 (PSGL-1), SNX17, and PODXL2.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Vestweber D, Blanks JE. 1999. Physiol. Rev. 79:181-213.
2. Ryan US, Worthington RE. 1992. Curr. Opin. Immunol. 4:33-37.
3. McEver RP, et al. 1989. J. Clin. Invest. 84:92-99.
4. Hsu-Lin S, et al. 1984. J. Biol. Chem. 259:9121-9126.
5. Stenberg PE, et al. 1985. J. Cell Biol. 101:880-886.
6. Hattori R, et al. 1989. J. Biol. Chem. 264:7768-7771.
7. Hahne M, et al. 1993. J. Cell Biol. 121:655-664.
8. Pan J, et al. 1998. J. Biol. Chem. 273:10058-10067.
9. Borsig L, et al. 2001. Proc. Natl. Acad. Sci. USA 99:2193-2198.
10. Bendas G, Borsig L. 2012. Int. J. Cell Biol. 2012:676731.
11. Xia L, et al. 2003. Blood 101:552-559.
12. Yao L, et al. 1996. J. Exp. Med. 184:81-92.
- Gene ID
- 20344 View all products for this Gene ID
- View information about P-Selectin on UniProt.org
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)