- Other Names
- Monocyte chemotactic protein 1 (MCP-1)
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- Product Citations
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CCL2, commonly known as MCP-1, is a member of the CC β chemokine family. It is widely expressed in endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β), and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of CCL2 in atherosclerosis. In addition, CCL2 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, CCL2 plays a key role in the regulation of MMPs during transmigration. CCL2 induces migration of MonoMac6 by induction of MMP2. CCL2 and CCL3/MIP-1α mediate firm adherence and (subsequent) transmigration of neutrophils via protein synthesis and secondary generation of leukotrienes and PAF, which in turn directly activate neutrophils in mouse cremaster muscle. CCL2 is induced from endothelial cells by MIF and from fetal skin fibroblasts by IL-17.Product Details
- Mouse MCP1, amino acids Gln24-Arg96 (Accession# NM_011333) was expressed in 293E cells.
- Molecular Mass
- The 73 amino acid recombinant protein has a predicted molecular mass of approximately 8.531 kD. The DTT-reduced protein migrates at approximately 12 kD and non-reduced protein migrates at approximately 13 kD by SDS-PAGE. The N-terminal amino acid is Glutamine.
- >98%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Mouse MCP-1/CCL2 chemoattracts human acute monocytic leukemia cell line THP-1 in a dose dependent manner. The ED50 is 0.3 to 2.0 ng/ml.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
(PubMed link indicates BioLegend citation)
- Mitchell LA, et al. 2013. Int Immunopharmacol. 15:357. PubMed.
- Product Citations
CCL2 (MCP-1) is secreted by fibroblasts, endothelial cells, smooth muscle cells, tumor cells, phytohemaglutinin stimulated mononuclear cells, TNF-α stimulated osteoblastic MC3T3-E1 cells.
- Monocytes, memory/ activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells.
- Ligand Receptor
- CCL2 mediates its cellular effects primarily through the CCR2 receptor.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction, Stem Cells
- Molecular Family
- Antigen References
1. Loberg RD, et al. 2007. Cancer Res. 67:9417.
2. Gregory JL, et al. 2006. J. Immunol. 177: 8072.
3. Qiu Z, et al. 2009. Immunobiology 214:835.
4. Reichel CA, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:1787.
5. McQuibban GA, et al. 2002. Blood 100:1160.
- Gene ID
- 20296 View all products for this Gene ID
- View information about CCL2 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.