- Other Names
- OMgp, Oligodendrocyte-myelin glycoprotein
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- Product Citations
OMG, also known as Oligodendrocyte myelin glycoprotein, is a glycosylphosphatidyl-inositol (GPI)-anchored protein expressed by oligodendrocytes, neurons, and astrocytes. OMG, along with Nogo (Neurite outgrowth inhibitor) and MAG (myelin-associated glycoprotein), belongs to a group of molecules located in central nervous system (CNS) myelin protein fractions with axon outgrowth inhibitory activity that prevents axonal regeneration after CNS injury. The neurite outgrowth inhibitory activities of these inhibitory molecules are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR). OMG also associates with Paired immunoglobulin-like receptor B (PirB). OMG contributes to the interactive process required for myelination in the central nervous system. OMG is also involved in the regulation of synaptic plasticity and is implicated in the inhibition of cell proliferation. The highly conserved leucine-rich repeat domain is required for inhibitory effect on both axon outgrowth and cell proliferation. Human OMG cDNA encodes a 440 amino acid residue GPI-anchored protein with a 24 amino acid signal peptide, eight leucine-rich repeats followed by five serine/threonine-rich repeats, and 23 amino acid propeptide. OMG has eleven potential N-glycosylation sites.Product Details
- Human OMG, amino acids Ile25-Asn420 (Accession# P23515) with C-terminal TGHHHHHHHHHHGGQ, was expressed in 293E cells.
- Molecular Mass
- The 411 amino acid recombinant protein has a predicted molecular mass of approximately 46.4 kD. The protein migrates at approximately 100-115 kD under both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Ile.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- >150 ng of protein, immobilized as a 3 µL droplet on a nitrocellulose-coated plate, is sufficient to significantly inhibit neurite outgrowth of E13 chick DRG neurons induced by laminin substrate.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
- OMG is expressed on the surface of oligodendrocytes, Purkinje cells of the cerebellum, pyramidal cells of the hippocampus, motor neurons of the brainstem, and the anterior horn cells of the spinal cord.
- Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system.
- Central nervous system (CNS) axons.
- Ligand Receptor
- NgR1 and PirB.
- Measured by the ability of immobilized protein to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Antigen References
1. Habib AA, et al. 1998. Oncogene 12:1525-31.
2. Wang KC, et al. 2002. Nature 417:941-944.
3. Kottis V, et al. 2002. J. Neurochem. 82:1566-1569.
4. Huang JK, et al. 2005. Science 310:1813-1817.
5. Atwal JK, et al. 2008. Science 322:967-970.
6. Raiker SJ, et al. 2010. J. Neurosci. 30:12432-12445.
7. Huang JY, et al. 2012. Brain Res. 1437:1-15.
8. Kempf A, et al. 2014. PLoS Biol. 12:e1001763.
- Gene ID
- 4974 View all products for this Gene ID
- View information about OMG on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.