ELISA MAX™ Deluxe Set Rat TNF-a

Pricing & Availability
Regulatory Status
Other Names
Tumor Necrosis Factor α (TNF-α) ELISA Kit, Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF)
Ave. Rating
Submit a Review
Product Citations
  • TNF-alpha_Rat_052511
Cat # Size Price Quantity Check Availability Save
438204 5 plates 278€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Please contact our sales representative for bulk pricing quotations.

TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing.  Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.  It has been shown that TNF-α plays a critical role in many physiological and pathological conditions including neurodegenerative diseases and cancer.

This kit is specifically designed for the accurate quantitation of Rat TNF-α from cell culture supernatant, serum, plasma, or other biological fluids.  It is designed to meet the increasing use of rat models in biomedical studies.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Rat TNF-α ELISA MAX™ Capture Antibody (200X)
  • Rat TNF-α ELISA MAX™ Detection Antibody (200X)
  • Rat TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Matrix Diluent A (For serum and plasma samples only)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Hajighorbani M, et al. 2017. Inflammopharmacology. 10.1007/s10787-017-0335-2. PubMed
  2. Garcia-Cortadella R, et al. 2021. Nat Commun. 0.646527778. PubMed
  3. Schmidt K, et al. 2017. Microvasc Res. 10.1016/j.mvr.2017.08.002. PubMed
  4. Sanchez TW, et al. 2020. Am J Reprod Immunol. 83:e13205. PubMed
  5. van der Merwe Y, et al. 2019. Sci Rep. 9:3482. PubMed
  6. Lai PH, et al. 2021. J Neuroinflammation. 18:35. PubMed
  7. Murugesan P, et al. 2016. J Infect Dis. 213: 532 - 540. PubMed
  8. Niu Q, et al. 2022. Life (Basel). 12:. PubMed
  9. Lotter J, et al. 2020. Front Psychiatry. 11:121. PubMed
2 pg/ml
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
24835 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 09.12.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account