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Purified Non-muscle Myosin Heavy Chain II-A Antibody (Previously Covance catalog# PRB-440P)

Pricing & Availability
Clone
Poly19098 (See other available formats)
Regulatory Status
RUO
Other Names
Myosin-9, NMMHC-A, MYH9 variant protein, myosin heavy chain 9, non-muscle myosin heavy chain A, cellular myosin heavy chain, type A, non-muscle myosin heavy polypeptide 9
Previously
Covance Catalog# PRB-440P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly19098_PURE_Myosin_Heavy_Chain_II-A_Antibody_WB_112817
Western blot analysis of cell lysates from A431, Jurkat, THP-1 (Human), Raw264.7 (Mouse) and UMR106 (Rat) cells using Non-muscle Myosin Heavy Chain II-A (Myosin-9) Rabbit primary antibody (clone Poly19098, 1:100 dilution, 1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_Poly19098_PURE_Myosin_Heavy_Chain_II-A_Antibody_WB_112817
    Western blot analysis of cell lysates from A431, Jurkat, THP-1 (Human), Raw264.7 (Mouse) and UMR106 (Rat) cells using Non-muscle Myosin Heavy Chain II-A (Myosin-9) Rabbit primary antibody (clone Poly19098, 1:100 dilution, 1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 2_Poly19098_PURE_Myosin_Heavy_Chain_II-A_Antibody_IF_112817
    Immunofluorescence of HepG2 cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Non-muscle Myosin Heavy Chain II-A (Myosin-9) primary antibody (clone19098). 2 µg/ml (1:250 dilution) Alexa Fluor® 594 (red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/40. Non-muscle Myosin Heavy Chain II-A antibody concentrations for (A, B) is 2 µg/ml (1:500 dilution), (C) is 1 µg/ml, (1:1000 dilution), (D) is 0.5 µg/ml. (1:500 dilution).
Cat # Size Price Quantity Check Availability Save
909802 25 µL 72€
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909801 100 µL 231€
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Description

Non-muscle Myosin Heavy Chain II-A is a member of the myosin superfamily. There are three forms of myosin II, called myosin IIA, myosin IIB and myosin IIC. These proteins are involved in cell motility, maintenance of cell shape, and cytokinesis. Each type of myosin II protein consists of two heavy chains and four light chains. The heavy chains each have two parts: a myosin head-like domain and a tail region. The head region interacts with actin, a protein that is important for cell movement and shape. The long tail region interacts with other proteins, including the tail regions of other myosin proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Cow
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against the peptide GKADGAEAKPAE corresponding to the C-terminus of human nonmuscle myosin heavy chain isoform A (MHC-A, NMHC IIA, Myosin-9).
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml (1:500 - 1:1000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml (1:500 - 1:1000 dilution) is recommended. The recommended usage for this polyclonal antibody may vary from lot to lot. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunofluorescence (IF).

*Predicted MW = 200 kD


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hirota Y, et al.. 2010. Development 137: 3037. (IF) PubMed
  2. Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
  3. Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
  4. Song J, et al. 2001. Mol Cell Neurosci. 17:624.
  5. Kelley CA, et al. 1996. J Cell Biol. 134:675.
  6. Kelley CA, et al. 1995. J Biol Chem. 270:1395.
  7. Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
Product Citations
  1. Chopra A, et al. 2018. Dev Cell. 44:87. PubMed
  2. Schell C, et al. 2018. Dev Cell. 47:741. PubMed
  3. Kokate SB, et al. 2022. Nat Commun. 13:6032. PubMed
  4. Priya R, et al. 2017. Mol Biol Cell. 28(1):12-20. PubMed
  5. Gebala V, et al. 2016. Nat Cell Biol. 10.1038/ncb3320. PubMed
  6. Schiffhauer ES, et al. 2019. J Cell Biol. 218:895. PubMed
  7. Trela E, et al. 2021. J Cell Biol. 220:. PubMed
  8. Aguilar-Cuenca R, et al. 2020. Current Biology. 30(13):2446-2458.e6. PubMed
  9. Indra I, et al. 2020. Cell Rep. 30:2820. PubMed
  10. Chen P, et al. 2016. J Biol Chem. 291: 7517-7526. PubMed
  11. Otterpohl K, et al. 2017. Physiol Rep.. 10.14814/phy2.13513. PubMed
  12. Masedunskas A, et al. 2018. J Cell Sci. 131:6. PubMed
  13. Sumigray KD et al. 2018. Developmental cell. 45(2):183-197 . PubMed
  14. Meddens M, et al. 2016. Nat Commun. 7:13127. PubMed
  15. Lehtimki JI, et al. 2021. eLife. 10:00. PubMed
  16. Jiu Y, et al. 2019. Curr Biol. 29:81. PubMed
  17. Eaton AF, et al. 2019. Mol Biol Cell. 30:2037. PubMed
  18. Rajakyl ä EK, et al. 2020. Cell Rep. 30:4266. PubMed
  19. Weienbruch K, et al. 2021. eLife. 0.416666666666667. PubMed
  20. Salomon J, et al. 2017. Nat Commun. 8:13998. PubMed
  21. Hirota Y, et al. 2010. Development. 137:3037-3046. PubMed
  22. Cheng C, et al. 2018. J Cell Sci. 131. PubMed
  23. Lechuga S, et al. 2022. FASEB J. 36:e22290. PubMed
  24. Makowska K, et al. 2015. Cell Rep. 13: 2118-2125. PubMed
  25. Yu H, et al. 2016. J Cell Sci. 129: 80 - 94. PubMed
  26. Wang F, et al. 2018. J Cell Biol. 217:4314. PubMed
  27. Taneja N, et al. 2019. Mol Biol Cell. 30:1051. PubMed
  28. Pfisterer SG, et al. 2017. Nat Commun. 8:14858. PubMed
  29. Fukuda SP, et al. 2017. Dev Growth Differ. 59:423. PubMed
  30. Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
  31. Cechova S, et al. 2018. J Am Soc Nephrol. 29:155. PubMed
  32. Zhovmer AS, et al. 2019. Mol Biol Cell. 30:1961. PubMed
  33. Kumari S, et al. 2020. EMBO J. 39:e102783. PubMed
  34. Naydenov NG, et al. 2022. Cells. 11:. PubMed
  35. Miller MR, et al. 2021. Mol Biol Cell. :mbcE21050268. PubMed
  36. Taneja N, et al. 2020. Cell Rep. 31:107477. PubMed
  37. Hu F, et al. 2022. iScience. 25:105514. PubMed
  38. Murakami K, et al. 2016. Diabetes. 65: 1255 - 1267. PubMed
  39. Le H, et al. 2016. Nat Cell Biol. 18: 864-875. PubMed
  40. Choi J, et al. 2019. J Cell Biol. 218:2277. PubMed
  41. Chen B, et al. 2022. Nat Methods. 19:1419. PubMed
  42. Zaman R, et al. 2021. J Cell Biol. 220:. PubMed
  43. Gaston C, et al. 2021. Nat Commun. 12:2226. PubMed
  44. Wang H, et al. 2008. J Cell Biol. 182:1171-1184. PubMed
  45. Van Itallie CM, et al. 2018. J Cell Sci. 131:. PubMed
  46. Ciuba K, et al. 2018. Sci Rep. 8:17670. PubMed
  47. Ning W, et al. 2020. Cell Stem Cell. 28(3):436-452.e5. PubMed
  48. Nguyen-Ngoc K, et al. 2017. J Cell Sci. 10.1242/jcs.208546. PubMed
RRID
AB_2734686 (BioLegend Cat. No. 909802)
AB_2565100 (BioLegend Cat. No. 909801)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4627 View all products for this Gene ID
UniProt
View information about Myosin-9 on UniProt.org
Go To Top Version: 7    Revision Date: 08.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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