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Purified F(ab')2 Goat anti-mouse IgM (μ chain) Antibody

Pricing & Availability
Clone
Poly21571 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
Poly21571_PUREAF_IgM_Antibody_1_090319
C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_1_090319
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD69 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_2_082919
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 3 days. Cells were surface stained with B220 APC and CD86 PE. Live cells were gated for analysis.
  • Poly21571_PUREAF_IgM_Antibody_3_082919
    C57BL/6 splenocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-mouse IgM (µ chain) antibody (clone Poly21571) (left) or unstimulated (right) for 2 minutes, fixed with Fixation buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, then stained with B220 Brilliant Violet 421™ and ZAP70 Phospho (Tyr319)/Syk (Tyr352) PE
Cat # Size Price Quantity Check Availability Save
157102 500 µg 221€
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Description

Poly21571 reacts with mouse IgM. It can be used in functional assays, such as B cell activation. It has minimum cross-reactivity with bovine proteins. The optimal working concentration should be determined empirically based on the specific assay of interest.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
Preparation
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. The solution contains no preservative; handle under aseptic conditions.
Application

Activ - Quality tested
Recommended Usage

This product was QC tested by stimulating C57BL/6 splenic B cells. The recommended working concentration is 10 - 20 µg/mL

RRID
AB_2814087 (BioLegend Cat. No. 157102)

Antigen Details

Cell Type
B cells
Biology Area
Immunology, Signal Transduction
Molecular Family
Adaptor Proteins
Gene ID
16019 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.29.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified F(ab')2 Goat anti-mouse IgM (μ chain)
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