Purified anti-XBP-1s Antibody

Pricing & Availability
Clone
9D11A43 (See other available formats)
Regulatory Status
RUO
Other Names
XBP-1 isoform2, Tax-responsive element-binding protein 5 (TREB5), XBP2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
9D11A43_PURE_XBP-1s_Antibody_1_100120
Total cell lysates (15 µg protein) from HepG2 cells treated without (-) or with (+) 300 nM thapsigargan for the indicated timepoints were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Xbp-1s antibodies (clones 9D11A43 and 143F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular weight ladder.
  • 9D11A43_PURE_XBP-1s_Antibody_1_100120
    Total cell lysates (15 µg protein) from HepG2 cells treated without (-) or with (+) 300 nM thapsigargan for the indicated timepoints were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Xbp-1s antibodies (clones 9D11A43 and 143F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular weight ladder.
  • 9D11A43_PURE_XBP-1s_Antibody_2_WB_062016
    Western blot analysis of HepG2 untreated (lane 1) cell, HepG2 treated with 300 nM of thapsigargin for overnight (lane 2), Raw264.7 untreated (lane 3) and Raw264.7 treated with 300 nM of thapsigargin for overnight (lane 4) using anti-XBP-1s antibody (clone 9D11A43). Purified anti-GAPDH antibody (Poly6314) was used as a loading control.
  • 9D11A43_PURE_XBP-1s_Antibody_3_070620
    HepG2 cells treated with thapsigargin (300nM, 16 hours) were stained with purified anti-XBP-1s (9D11A43) antibody, followed by staining with DyLight 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 9D11A43_PURE_XBP-1s_Antibody_4_070620
    Chromatin Immunoprecipitations (ChIP) were performed using fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2.0 µg/mL, 8 hr). ChIP was performed with 10.0 µg of chromatin and 2.0 µg of purified anti- XBP-1s antibody (clone 9D11A43) or Go-ChIP-Grade™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400183). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human DNAJB9 exon 1 region and the human α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
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658802 100 µg 199€
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Description

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of the human HLA-DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 is upregulated as part of the ER stress response, also known as the unfolded protein response.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial mouse XBP-1s recombinant protein (162-267aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For ChIP application, use 2 µg of antibody and 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone 9D11A43 recognizes spliced XBP-1 (XBP-1s/isoform 2), which is 371 amino acids and migrates around 55 kD on the SDS-PAGE. 

Additional Product Notes

Using this antibody at a ratio higher than 1:5 (µg antibody per ug of chromatin) will increase background

Product Citations
  1. Sundaram A et al. 2017. eLife. 6 pii: e27187. PubMed
  2. Saito A et al. 2017. Journal of neurochemistry. 144(1):35-49 . PubMed
  3. Bezu L, et al. 2018. Cell Death Differ. 25:1375. PubMed
RRID
AB_2562960 (BioLegend Cat. No. 658802)

Antigen Details

Distribution

Nucleus.

Function
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
MHC Antigens, Nuclear Markers
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 12.15.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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