Western blot of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system
Western blot of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system
IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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