Purified anti-rat CD252 (OX-40 Ligand) Antibody

Pricing & Availability
ATM-2 (See other available formats)
Regulatory Status
Other Names
OX-40 Ligand, CD134L, TNFSF4
Mouse IgG1, κ
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Product Citations
PMA-stimulated (2 days) Lou rat splenocytes stained with ATM-2 biotin, then detected with Sav-PE
  • ATM-2
    PMA-stimulated (2 days) Lou rat splenocytes stained with ATM-2 biotin, then detected with Sav-PE
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200501 50 µg 71€
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OX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4), also known as CD252 or CD134L. It is expressed on dendritic cells, peritoneal macrophages, and activated B cells. The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation. The ATM-2 antibody is able to block the costimulatory function of OX-40L.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
ACI-S1 rat T-cell lymphoma cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).

Application References

(PubMed link indicates BioLegend citation)
  1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
AB_2571881 (BioLegend Cat. No. 200501)

Antigen Details

TNF superfamily, 22 kD
Dendritic cells, activated B cells
T cell dependent humoral responses
OX40 (CD134)
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.
3. Akiba, H., et al. 2000. J. Exp. Med. 191:375.

Gene ID
22164 View all products for this Gene ID
View information about CD252 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03.17.2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Purified anti-rat CD252 (OX-40 Ligand)


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