- TW7-20B9 (See other available formats)
- Other Names
- Transforming growth factor-beta 1 (TGF-b1), Latency Associated Peptide (LAP), TGFB1, DPD1
- Mouse IgG1, κ
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- Product Citations
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Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associated peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.Product Details
- Antibody Type
- Host Species
- Mouse Tgfb1-transduced P3U1 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, WB, Neut - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone TW7-20B9 has been reported to not cross-react with human LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 This antibody recognizes recombinant LAP, latent TGF-ß, and pro-TGF-ß.
Additional reported applications (for relevant formats) include: Western blotting1, immunoprecipitation1, and neutralization2. TW7-20B9 is able to neutralize certain unconventional T cell-derived forms of TGF-ß activity, but not the distinct 25 kDa free form of TGF-ß.2
(PubMed link indicates BioLegend citation)
- Oida T, et al. 2010. PLoS ONE (FC, IP, WB)
- Oida T, et al. 2011. PLoS ONE 6:e18365. (Neut)
- Tu Z, et al. 2012. Invest Ophthalmol Vis Sci. 53:959. PubMed
- Product Citations
AB_10717504 (BioLegend Cat. No. 141302)
- Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Many cell types, highly expressed on activated Tregs and platelets
- TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.
- TGF-β receptors
- Cell Type
- Dendritic cells, Platelets, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
1. Oida T, et al. 2010. PLoS
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.
- Gene ID
- 21803 View all products for this Gene ID
- View information about LAP on UniProt.org
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