- TRFK5 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.Product Details
- Verified Reactivity
- Mouse, Human
- Reported Reactivity
- Guinea Pig
- Antibody Type
- Host Species
- Partially-purified T cell clone supernatant
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
CyTOF® - Verified
IHC, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with TRFK4 (Cat. # 504402). To obtain a linear standard curve, serial dilutions of human IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with JES1-5A10 (Cat. # 501002). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.
Neutralization1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
- Swain, S., et al. 1990. J. Immunol. 145:3796.
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
- Karulin, A., et al. 2000. J. Immunol. 164:1862.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
- Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
- Product Citations
AB_315325 (BioLegend Cat. No. 504301)
AB_315326 (BioLegend Cat. No. 504302)
- Cytokine; dimer; 40-50 kD (Mammalian)
- Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
- Cell Sources
- Mast cells, T cells, eosinophils
- Cell Targets
- Eosinophils, B cells
- Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.
- Gene ID
- 16191 View all products for this Gene ID 3567 View all products for this Gene ID
- View information about IL-5 on UniProt.org
|APC anti-mouse/human IL-5||TRFK5||ICFC|
|PE anti-mouse/human IL-5||TRFK5||ICFC|
|Purified anti-mouse/human IL-5||TRFK5||ELISA Capture, CyTOF®, IHC, WB|
|Purified anti-mouse/human IL-5 (Maxpar® Ready)||TRFK5||ELISA Capture, CyTOF®|
|Brilliant Violet 421™ anti-mouse/human IL-5||TRFK5||ICFC|
|Ultra-LEAF™ Purified anti-mouse/human IL-5||TRFK5||ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC, IHC, WB|
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