Purified anti-mouse GITR Ligand Antibody

Pricing & Availability
Clone
YGL 386 (See other available formats)
Other Names
AITRL, TNFSF18, TL6, GITRL
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
120302 200 µg 200€
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Description

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells and endothelial cells. The expression of GITRL on dendritic cell surface can be transiently up-regulated and then rapidly down-regulated by LPS stimulation. GITRL binds to its receptor, GITR, to co-stimulate T cell activation, proliferation, and cytokine production, and inhibit the suppressive functions of CD4+CD25+ Treg cells, as well as induce macrophages activation. GITR-GITRL interaction has been implicated as an essential regulator of immune response to self antigens and phathogens.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse GITRL protein and Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059
  2. Lu LF, et al. 2005. J. Immunol. 175:1651
  3. Esparza EM and RH Arch. 2005. J. Immunol. 174:7875
  4. Ray A, et al. 2012. J. Immunol. 188:3188. PubMed.
RRID
AB_2207249 (BioLegend Cat. No. 120302)

Antigen Details

Structure
TNF superfamily member
Distribution

B cells, macrophages, dendritic cells, endothelial cells.

Function
Bind GITR receptor to co-stimulate T cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand/Receptor
GITR (TNFRSF18)
Cell Type
B cells, Dendritic cells, Endothelial cells, Macrophages, Tregs
Biology Area
Immunology
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.

Gene ID
240873 View all products for this Gene ID
UniProt
View information about GITRL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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