Loading...

Purified anti-mouse CD86 Antibody

Pricing & Availability
Clone
GL-1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
GL-1_Purified_090707
C57BL/6 mouse splenocytes stained with purified GL-1,followed by anti-rat IgG FITC
  • GL-1_Purified_090707
    C57BL/6 mouse splenocytes stained with purified GL-1,followed by anti-rat IgG FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
105001 50 µg 44€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
105002 500 µg 111€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  3. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  4. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  6. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  7. Zhang P, et al. 2007. J Immunol. 178:474. PubMed
  8. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  9. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  10. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  11. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  12. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  13. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  14. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  15. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  17. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  18. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  19. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  20. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  21. Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed
  22. Vander B, et al. 2017. J Cell Biol. 10.1083/jcb.201512012. PubMed
  23. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  24. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  25. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  26. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  27. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  28. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  29. Zhong Z, et al. 2022. Bioact Mater. 10:195. PubMed
  30. Klinger M, et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293:R677. PubMed
RRID
AB_313144 (BioLegend Cat. No. 105001)
AB_313145 (BioLegend Cat. No. 105002)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account