Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
W20119E (See other available formats)
Regulatory Status
Other Names
Rat IgG2b, κ
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Product Citations
TE-71 cells were stained with purified anti-mouse CD326 (Ep-CAM) (clone W20119E) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W20119E_PURE_CD326_Antibody_1_081922
    TE-71 cells were stained with purified anti-mouse CD326 (Ep-CAM) (clone W20119E) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W20119E_PURE_CD326_Antibody_2_081922
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD326 (Ep-CAM) (clone W20119E) and Alexa Fluor® 647 anti-mouse Podoplanin (clone PMab-1) (green) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG2b (clone MRG2b-85) (red) for an hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X object.
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112202 100 µg 84€
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Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

AB_2924452 (BioLegend Cat. No. 112202)

Antigen Details

40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Mediates calcium-independent homophilic cell-cell adhesion
CD326 displays hemophilic binding.
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
View information about CD326 on UniProt.org

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Go To Top Version: 1    Revision Date: 08.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.


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