- 6D5 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse CD19-expressing K562 human erythroleukemia cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence7.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
- Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
- Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
AB_2562806 (BioLegend Cat. No. 115547)
- Ig superfamily, associates with CD21 and CD81, 95 kD
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Modulates B cell activation and differentiation
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- View information about CD19 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD19
Biotin anti-mouse CD19
FITC anti-mouse CD19
PE anti-mouse CD19
PE/Cyanine5 anti-mouse CD19
Purified anti-mouse CD19
PE/Cyanine7 anti-mouse CD19
Alexa Fluor® 488 anti-mouse CD19
Alexa Fluor® 647 anti-mouse CD19
Pacific Blue™ anti-mouse CD19
Alexa Fluor® 700 anti-mouse CD19
APC/Cyanine7 anti-mouse CD19
PerCP anti-mouse CD19
PerCP/Cyanine5.5 anti-mouse CD19
Alexa Fluor® 594 anti-mouse CD19
Brilliant Violet 421™ anti-mouse CD19
Brilliant Violet 570™ anti-mouse CD19
Brilliant Violet 605™ anti-mouse CD19
Brilliant Violet 650™ anti-mouse CD19
Brilliant Violet 785™ anti-mouse CD19
Brilliant Violet 510™ anti-mouse CD19
Purified anti-mouse CD19 (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse CD19
Brilliant Violet 711™ anti-mouse CD19
APC/Fire™ 750 anti-mouse CD19
TotalSeq™-A0093 anti-mouse CD19
Brilliant Violet 750™ anti-mouse CD19
TotalSeq™-B0093 anti-mouse CD19
Spark Blue™ 550 anti-mouse CD19
Spark NIR™ 685 anti-mouse CD19
TotalSeq™-C0093 anti-mouse CD19
Ultra-LEAF™ Purified anti-mouse CD19
PE/Fire™ 640 anti-mouse CD19 Antibody
Spark YG™ 581 anti-mouse CD19
APC/Fire™ 810 anti-mouse CD19
Spark YG™ 570 anti-mouse CD19
Spark Blue™ 574 anti-mouse CD19 Antibody