Purified anti-mouse CD183 (CXCR3) Antibody

Pricing & Availability
Clone
CXCR3-173 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype
Armenian Hamster IgG
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Product Citations
publications
CXCR3-173_Pure_071408.jpg
C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
  • CXCR3-173_Pure_071408.jpg
    C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
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126502 200 µg 80,00€
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Description

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse N-terminus of CXCR3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Krug A, et al. 2002. J. Immunol. 169:6079.
  2. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
  3. Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
  4. Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
  5. Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
  6. Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
  7. Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations
  1. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  2. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  3. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  4. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  5. Dürr C, et al. 2010. Cancer Res. 70:10170. PubMed
  6. Seung E, et al. 2011. J Immunol. 186:6830. PubMed
  7. Haworth O, et al. 2011. J Immunol. 186:6129. PubMed
  8. Martin F, et al. 2011. Infect Immun. 79:1898. PubMed
  9. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
RRID
AB_1027635 (BioLegend Cat. No. 126502)

Antigen Details

Distribution

T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors

Function
Essential in T cell recruitment to sites of inflammation
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID
12766 View all products for this Gene ID
UniProt
View information about CD183 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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