Purified anti-Keratin 10 Antibody

Pricing & Availability
Clone
Poly19054 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type I cytoskeletal 10, CK-10, keratin-10, cytokeratin 10
Isotype
Rabbit Polyclonal
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Product Citations
publications
Poly19054_PURE_Keratin10_Antibody_1_IHCP_062216
IHC staining of anti-Keratin 10 antibody (Poly19054) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
  • Poly19054_PURE_Keratin10_Antibody_1_IHCP_062216
    IHC staining of anti-Keratin 10 antibody (Poly19054) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
  • Poly19054_PURE_Keratin10_Antibody_2_WB_062216
    Western Blot of anti-Keratin 10 antibody (Poly19054). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature, followed by incubation with a fluorescent labeled goat anti-rabbit secondary antibody.
  • Poly19054_PURE_Keratin10_Antibody_3_WB_011918
    Total lysates (15 µg protein) from Raw264.7 (Mouse Low expression negative control) and Mouse skin (Positive control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Cytokeratin 10 antibody, clone Poly 19054. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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905403 25 µg 60,00€
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905404 100 µg 196,00€
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Description

Keratin 10 is a member of the type 1 (acidic) cytokeratin family. Keratins, in general, are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Cross-Reactivity: Dog, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 10 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams JR, et al. 2011. Cancer Res. 71:2706.
  2. Lacroix M, et al. 2010. Proc. Natl. Acad. Sci. USA 107:21076-21081. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature 410:710.
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  5. Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
  6. Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Nishiguchi MA et al. 2018. Cell reports. 24(13):3383-3392 . PubMed
  2. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  3. Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed
  4. Koenig U, et al. 2019. Autophagy. :1. PubMed
  5. Lough KJ, et al. 2019. Elife. 8:e49249. PubMed
  6. Byrd KM, et al. 2020. Cell Stem Cell. 25(6):814-829.e6.. PubMed
  7. Wong W, et al. 2020. PLoS One. 15:e0241617. PubMed
  8. Cai EY, et al. 2020. Cell Stem Cell. 27(2):270-283.e7. PubMed
RRID
AB_2749902 (BioLegend Cat. No. 905403)
AB_2616955 (BioLegend Cat. No. 905404)

Antigen Details

Structure
Keratin 10 is a 59 kD protein.
Distribution

Cytoskeleton, extracellular, and nucleus.

Function
Keratin 10 generally associates with keratin 1. Keratin 10 mutations are associated with epidermolytic hyperkeratosis.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3858 View all products for this Gene ID
UniProt
View information about Keratin 10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09.25.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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