- Clone
- A15153G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood leukocytes were stained with CD3 FITC and purified anti-human TIGIT (clone A15153G) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by PE goat anti-mouse IgG. Data shown were gated on lymphocyte population. -
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Human PBMCs were labeled with CFSE. Cells were cultured in 6-well plate wells that were pre-coated with anti-CD3 plus mouse IgG2a isotype control (open histogram) or with anti-CD3 plus anti-human TIGIT (clone A15153G) (filled histogram). After four days of incubation, cells were harvested and stained for surface CD3. Data shown are SFSE profiles of CD3+ cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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372702 | 100 µg | 81€ |
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human TIGIT.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
Additional reported applications (for the relevant formats) include: Blocking1. -
Application References
(PubMed link indicates BioLegend citation) -
- Stamm H, et al. 2018. Oncogene. Pubmed
- Product Citations
-
- RRID
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AB_2632714 (BioLegend Cat. No. 372702)
Antigen Details
- Structure
- 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
- Distribution
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Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
- Function
- Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
- Ligand/Receptor
- CD155 (PVR), CD112 (PVRL2, NECTIN-2).
- Cell Type
- NK cells, T cells, Tfh, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
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1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923. - Gene ID
- 201633 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TIGIT (VSTM3)
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APC/Fire™ 750 anti-human TIGIT (VSTM3)
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APC anti-human TIGIT (VSTM3)
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PE anti-human TIGIT (VSTM3)
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Brilliant Violet 421™ anti-human TIGIT (VSTM3)
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Brilliant Violet 605™ anti-human TIGIT (VSTM3)
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PE/Dazzle™ 594 anti-human TIGIT (VSTM3)
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PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)
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PE/Cyanine7 anti-human TIGIT (VSTM3)
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Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)
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Biotin anti-human TIGIT (VSTM3)
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Alexa Fluor® 647 anti-human TIGIT (VSTM3)
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TotalSeq™-A0089 anti-human TIGIT (VSTM3)
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TotalSeq™-B0089 anti-human TIGIT (VSTM3)
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TotalSeq™-C0089 anti-human TIGIT (VSTM3)
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KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)
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APC/Cyanine7 anti-human TIGIT (VSTM3)
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Brilliant Violet 510™ anti-human TIGIT (VSTM3)
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Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody
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TotalSeq™-D0089 anti-human TIGIT (VSTM3)
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Brilliant Violet 711™ anti-human TIGIT (VSTM3)
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PE/Fire™ 640 anti-human TIGIT (VSTM3)
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PE/Fire™ 810 anti-human TIGIT (VSTM3)
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PE/Cyanine5 anti-human TIGIT (VSTM3)
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PerCP/Fire™ 806 anti-human TIGIT (VSTM3)
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PerCP/Fire™ 780 anti-human TIGIT (VSTM3)
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