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Purified anti-human TCR γ/δ Antibody

Pricing & Availability
Clone
B1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ, γ/δ TCR, TCR-γ/δ
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B1_Pure_021009.jpg
Human peripheral blood lymphocytes stained with purified B1, followed by anti-mouse IgG FITC
  • B1_Pure_021009.jpg
    Human peripheral blood lymphocytes stained with purified B1, followed by anti-mouse IgG FITC
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331202 100 µg 92€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a subset of T cells found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone B1 is also known as clone B1.1.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3 and paraffin-embedded sections5, in vitro blocking, and spatial biology (IBEX)8,9. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. Nos. 331235 and 331236).

Application References

(PubMed link indicates BioLegend citation)
  1. Rodriguez-Gago M, et al. 2001. Transplantation. 72:503.
  2. Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC)
  3. Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC)
  4. Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC)
  5. Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC)
  6. Correia DV, et al. 2011. Blood. 118:992. (Block)
  7. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  3. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  4. Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
  5. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  6. Hu Y, et al. 2022. Front Oncol. 12:845765. PubMed
  7. Fenton TM, et al. 2020. Immunity. 52(3):557-570. PubMed
  8. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  9. Ardain A, et al. 2019. Nature. 570:528. PubMed
  10. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  11. Albertsson AM, et al. 2018. Am J Pathol. 188:757. PubMed
  12. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_1089222 (BioLegend Cat. No. 331202)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex
Distribution

T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum

Function
Antigen recognition
Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I, CD1 molecule
Cell Type
Epithelial cells, T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429.
2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335.
3. Uyemura K, et al. 1991. J. Exp. Med. 174:683.
4. Spada FM, et al. 2000. J. Exp. Med. 191:907.

Gene ID
6964 View all products for this Gene ID 6965 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 08.17.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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