Purified anti-human IL-3 Antibody

Pricing & Availability
BVD8-3G11 (See other available formats)
Regulatory Status
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Rat IgG1, κ
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Product Citations
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500502 500 µg 210€
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IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Yeast-expressed, recombinant human IL-3
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested
WB, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting4 and immunoprecipitation4.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
  2. Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
  3. Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
  4. Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
  5. Mahanty S, et al. 1992. J. Immunol. 148:3567.
AB_315103 (BioLegend Cat. No. 500502)

Antigen Details

Cytokine; 14-30 kD (Mammalian)
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
IL-3R, α subunit (CD123), common β subunit (CDw131).
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.

Upregulated by T cell stimulation; downregulated by glucocorticoids.
Gene ID
3562 View all products for this Gene ID
View information about IL-3 on UniProt.org

Related FAQs

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Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-3 BVD8-3G11 ELISA Capture, IP, WB
Ultra-LEAF™ Purified anti-human IL-3 BVD8-3G11 Block, IP, WB
Go To Top Version: 1    Revision Date: 11.30.2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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