- M4509E12 (See other available formats)
- Regulatory Status
- Other Names
- IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B, Interferon Alpha 2
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α; therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 2.0 - 8.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-α2 recombinant protein ranging from 12.5 to 800 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The pure M4509E12 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BL25372 antibody (Cat. No. 537204) as the capture antibody and Recombinant Human IFN-a2 (ELISA Std.) (Cat. No. 592709)
AB_2783346 (BioLegend Cat. No. 537102)
IFN-α is ubiquitously expressed.
- IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
- A variety of cells express the IFNα receptor (IFNAR).
- IFNAR complex has two components, IFNAR1 and IFNAR2.
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Soluble Receptors
- Antigen References
- Bose A and Baral R. 2007. Immunol Lett. 108:68.
- Zhao W, et al. 2008. J Immunol. 180:5483.
- Badiger R, et al. 2012. Plos One. 7:e46779.
- Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485.
- Becker-Merok A, et al. 2013. Lupus. 22:155.
- Mukherjee KK, et al. 2012. Indian J Med Res. 136:54.
- Gene ID
- 3440 View all products for this Gene ID
- View information about IFN-alpha2 on UniProt.org
|Purified anti-human IFN-α2||M4509E12||ELISA Capture|
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