Purified anti-human CD261 (DR4, TRAIL-R1) Antibody

Pricing & Availability
Clone
DJR1 (See other available formats)
Workshop
HCDM listed
Other Names
TRAIL-R1, Apo-2, CD261, TNFRSF10A
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
DJR1_Purified_072007
Human DR4 transfected cells stained with purified DJR1, followed by anti-mouse IgG FITC
  • DJR1_Purified_072007
    Human DR4 transfected cells stained with purified DJR1, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Avail. Save
307201 25 µg 68€
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307202 100 µg 140€
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Description

DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of DR4-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307204) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Uno K, et al. 2003. Blood 101:3658.
  2. Sato K, et al. 2005. J. Immunol. 174:4025.
  3. Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed
  4. Chen KF, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed
  5. Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
  6. Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
Product Citations
  1. Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed
  2. Chen K, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed
  3. Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
  4. Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
RRID
AB_314665 (BioLegend Cat. No. 307201)
AB_314666 (BioLegend Cat. No. 307202)

Antigen Details

Structure
TNFR superfamily, 56 kD
Distribution

Activated T cells, tumor cells

Function
Induces apoptosis
Ligand/Receptor
TRAIL
Cell Type
T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Macfarlane M. 2003. Toxicol. Lett. 139:89.
2. Baetu T, et al. 2002. Cytokine Growth Factor Rev. 13:199.
3. Degli-Esposti M. 1999. J. Leukoc. Biol. 65:535.

Gene ID
8797 View all products for this Gene ID
UniProt
View information about CD261 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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