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Purified anti-human CD2 Antibody

Pricing & Availability
Clone
RPA-2.10 (See other available formats)
Regulatory Status
RUO
Workshop
IV T085
Other Names
LFA-2, T11, SRBC-R
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA_2dot10_Purified_080204
Human peripheral blood lymphocytes stained with purified RPA-2.10, and detected with anti-mouse IgGs FITC
  • RPA_2dot10_Purified_080204
    Human peripheral blood lymphocytes stained with purified RPA-2.10, and detected with anti-mouse IgGs FITC
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300202 100 µg 70€
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Description

CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
  3. Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
  4. Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
  5. Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
  6. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  3. Thümmler K, et al. 2010. J Leukoc Biol. 88:1041. PubMed
  4. Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
  5. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  6. Colleen S McGary et al. 2017. Immunity. 47(4):776-788 . PubMed
  7. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  8. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  9. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  10. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  11. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  12. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_314026 (BioLegend Cat. No. 300202)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution

T cells, NK cells, thymocytes, and thymic B cell subsets

Function
T cell activation, adhesion
Ligand/Receptor
CD58 (LFA-3), CD48, CD59, CD15
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.

Gene ID
914 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08.18.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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