Purified anti-human CD177 Antibody

Pricing & Availability
Clone
MEM-166 (See other available formats)
Workshop
HCDM listed
Other Names
Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
MEM-166_Pure_020708
Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
  • MEM-166_Pure_020708
    Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
Cat # Size Price Quantity Avail. Save
315802 100 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IF, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.

Application References

(PubMed link indicates BioLegend citation)
  1. Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press.
  2. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed
  3. Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed
  4. von Vietinghoff S, et al. 2007. Blood 109:4487. (IF)
  5. Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)
Product Citations
  1. Vietinghoff S, et al. 2007. Blood. 109:4487. PubMed
  2. Korkmaz B, et al. 2008. J Biol Chem. 283:35976. PubMed
RRID
AB_2072605 (BioLegend Cat. No. 315802)

Antigen Details

Structure
uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution

Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)

Function
Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification
Glycosylated
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.

Gene ID
57126 View all products for this Gene ID
UniProt
View information about CD177 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.18.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account