Purified anti-human CD172b (SIRPβ) Antibody

Pricing & Availability
Clone
B4B6 (See other available formats)
Workshop
HCDM listed
Other Names
SIRPb, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B4B6_Pure_030308
Human peripheral blood granulocytes stained with purified B4B6, followed by biotinylated anti-mouse IgG and Sav-PE
  • B4B6_Pure_030308
    Human peripheral blood granulocytes stained with purified B4B6, followed by biotinylated anti-mouse IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
323902 100 µg 80€
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Description

The B4B6 antibody recognizes human CD172b also known as signal-regulatory protein beta, and SIRP-beta. CD172b is a member of the signal regulatory protein family and immunoglobulin superfamily. CD172b contains a single pass transmembrane region with immunoglobulin-like domains and unlike CD172a, this receptor lacks a cytoplasmic SHP-2 binding domain. CD172b is a disulfide-linked homodimer with a predicted molecular weight of approximately 43 kD. Two isoforms of CD172b are produced by alternative splicing. CD172b is expressed on myeloid cells including monocytes and dendritic cells where it functions in the negative regulation of tyrosine-kinase coupled signaling processes. CD172b has been shown to regulate neutrophil transepithelial migration. CD172b interacts with DAP12 and Syk kinase (through TYROBP). The B4B6 antibody has been shown to be useful for flow cytometry.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with SIRPbeta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Seiffert M, et al. 2001. Blood 97:2741.
Product Citations
  1. Dutertre CA et al. 2019. Immunity. 51(3):573-589 . PubMed
Publication Library
RRID
AB_2301799 (BioLegend Cat. No. 323902)

Antigen Details

Structure
Member of the signal regulatory protein family and immunoglobulin superfamily. Single pass transmembrane receptor with immunoglobulin-like domains; lacks cytoplasmic SHP-2 binding domain. Disulfide-linked homodimer, predicted MW ~43 kD.
Distribution

Myeloid cells, monocytes, dendritic cells

Function
Negative regulation of tyrosine-kinase coupled signaling processes. Regulates neutrophil transepithelial migration
Interaction
DAP12 and Syk (through TYROBP)
Cell Type
Monocytes, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kharitonenkov A, et al. 1997. Nature 386:181.
2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147.

Gene ID
10326 View all products for this Gene ID
UniProt
View information about CD172b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.25.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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