- 6H6 (See other available formats)
- Regulatory Status
- Other Names
- IL-3Rα, IL-3 Receptor alpha
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Human IL-3Rα transfected COS cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
- Product Citations
AB_2562823 (BioLegend Cat. No. 306027)
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Hematopoietic cell proliferation, differentiation
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- View information about CD123 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD123
PE anti-human CD123
Purified anti-human CD123
PE/Cyanine5 anti-human CD123
PE/Cyanine7 anti-human CD123
APC anti-human CD123
FITC anti-human CD123
PerCP/Cyanine5.5 anti-human CD123
Brilliant Violet 421™ anti-human CD123
Brilliant Violet 650™ anti-human CD123
Brilliant Violet 510™ anti-human CD123
Alexa Fluor® 647 anti-human CD123
Brilliant Violet 605™ anti-human CD123
Purified anti-human CD123 (Maxpar® Ready)
Brilliant Violet 711™ anti-human CD123
Brilliant Violet 785™ anti-human CD123
PE/Dazzle™ 594 anti-human CD123
Alexa Fluor® 488 anti-human CD123
PE/Cyanine7 anti-human CD123
TotalSeq™-A0064 anti-human CD123
Alexa Fluor® 700 anti-human CD123
APC/Fire™ 750 anti-human CD123
Pacific Blue™ anti-human CD123
TotalSeq™-C0064 anti-human CD123
TotalSeq™-B0064 anti-human CD123
TotalSeq™-D0064 anti-human CD123
PerCP anti-human CD123
GMP PE/Cyanine7 anti-human CD123
APC/Fire™ 810 anti-human CD123 Antibody