- 2G2.3.5 (See other available formats)
- Regulatory Status
- Other Names
- Ubiquitin-Activating Enzyme E1, Ubiquitin-Like Modifier Activating Enzyme 1, UBE1, A1S9T, GXP1, SMAX2, 2G.2.3.5, 2G2.3-5
- Mouse IgG1
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- Product Citations
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The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Mixture of human and mouse E1 protein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
AB_2565603 (BioLegend Cat. No. 932002)
- 1058 amino acids with a predicted molecular weight of approximately 118 kD.
Ubiquitous, Eukaryotic cells.
- This protein catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation.
- Biology Area
- Cell Biology, DNA Repair/Replication, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Molecular Family
- Protein Modifiers
- Antigen References
1. Xu W, et al. 2013. Curr. Pharm. Des. 18:3201.
2. Neutzner M, et al. 2012. Essays Biochem. 52:37.
3. Schulman BA, et al. 2009. Nat. Rev. Mol. Cell Biol. 10:319.
4. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.
- Gene ID
- 7317 View all products for this Gene ID
- View information about E1 Ubiquitin Activating Enzyme on UniProt.org
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