Purified anti-DDX5 Antibody

Pricing & Availability
Clone
14B9A58 (See other available formats)
Other Names
Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
14B9A58_DDX5_Purified_1_WB_062518
Whole cell lysates (15 µg protein) from HeLa, HEL, MOLT-4, and RAW264.7 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-DDX5 antibody, clone 14B9A58. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • 14B9A58_DDX5_Purified_1_WB_062518
    Whole cell lysates (15 µg protein) from HeLa, HEL, MOLT-4, and RAW264.7 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-DDX5 antibody, clone 14B9A58. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • 14B9A58_DDX5_Purified_2_WB_062518
    Total cell lysate from 293T cells (lane 1) or NIH-3T3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX5 (clone 14B9A58) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 14B9A58_DDX5_Purified_3_ICC_062518
    HeLa cells were stained with purified anti-DDX5 (14B9A58) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
661302 100 µg 180€
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Description

DDX5, also named RNA helicase p68, is a prototypic member of the DEAD (Asp-Glu-Ala-Asp) box family. DDX5 has been shown to be involved in the processes requiring the alteration of RNA structures, such as the maturation of pre-mRNA, rRNA, and miRNA. DDX5 also plays important helicase-independent roles in transcriptional regulation. DDX5 can act as a transcriptional coactivator of the androgen receptor (AR), estrogen receptor α (ERα), tumor suppressor p53, MyoD, RUNX2, and β-catenin, which are involved in the regulation of cell proliferation, differentiation, and tumorigenesis. DDX5 has been shown to be up-regulated in various cancers, including breast cancer, prostate cancer, and colorectal cancer, suggesting that DDX5 may participate in tumor development. DDX5 can also act as a transcriptional repressor through interacting with HDAC1.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX5 recombinant protein (482-614 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2563942 (BioLegend Cat. No. 661302)

Antigen Details

Structure
614 amino acids, predicted molecular weight of 69 kD. Contains a DEAD box domain, a helicase domain, and a transactivation domain.
Distribution

Nucleus.

Function
DDX5 is an ATP-dependent RNA helicase, regulating RNA processing and ribosome biogenesis. DDX5 is also involved in transcription by acting as a transcriptional coactivator or a repressor.
Interaction
DDX5 interacts with DDX17, RBM4, AGO1, AGO2, ESR1, p300, RUNX2, and HDAC1.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Iyer RS, et al. 2014. Cell Cycle. 13:1413.
2. Samaan S, et al. 2014. Nucleic Acids Res. 42:2197.
3. Fuller-Pace FV. 2013. Biochim. Biophys. Acta. 1829:756.
4. Wang D, et al. 2012. Mol. Cell Proteomics. 11:M111.011932.
5. Saporita AJ, et al. 2011. Cancer Res. 71:6708.
6. Clark EL, et al. 2008. Cancer Res. 68:7938.

Gene ID
1655 View all products for this Gene ID
UniProt
View information about DDX5 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06.25.2018

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