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Purified anti-CRISPR (CAS9) Antibody

Pricing & Availability
Clone
7A9 (See other available formats)
Regulatory Status
RUO
Other Names
CRISPR associated protein 9, SpyCas9
Isotype
Mouse IgG1, κ
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Product Citations
publications
7A9_PUREAF_CRISPR_Antibody_FC_041015
HeLa cells expressing Tet-inducible Flag-tagged S. pyogenes Cas9 were seeded onto glass cover slips and induced with 1.5 µg/ml Doxycyclin for 30 hours. Cells were fixed in 3.7% formaldehyde in PBS, permeablized, and stained with anti-Cas9 (Clone 7A9) antibody at 4°C for 24 hours. After incubation with donkey anti-mouse Alexa Fluor® 488, the nuclei were counter-stained with Hoechst 33342.
  • 7A9_PUREAF_CRISPR_Antibody_FC_041015
    HeLa cells expressing Tet-inducible Flag-tagged S. pyogenes Cas9 were seeded onto glass cover slips and induced with 1.5 µg/ml Doxycyclin for 30 hours. Cells were fixed in 3.7% formaldehyde in PBS, permeablized, and stained with anti-Cas9 (Clone 7A9) antibody at 4°C for 24 hours. After incubation with donkey anti-mouse Alexa Fluor® 488, the nuclei were counter-stained with Hoechst 33342.
  • 7A9_PUREAF_CRISPR_Antibody_WB_041015
    HeLa cells were transiently transfected with Flag-tagged S. pyogenes Cas9 and cells are lysed in hot Laemmli buffer at 30 hours post transfection. Equal amounts of untransfected and transfected cell lysates were separated by 7.5% SDS-PAGE, transferred to nitrocellulose membrane, and incubated at 4°C with the indicated antibodies. Cas9 was used at 0.5 µg/ml.
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844302 25 µg 72€
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844301 100 µg 203€
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Description

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 interrogates and cleaves foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking if it is complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
S. Pyogenes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant S. pyogenes Cas9 Protein (N-terminal 250 amino acids) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
WB, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested working dilution is 1.0 - 10 µg/ml. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Usman WM, et al. 2018. Nat Commun. 9:278. PubMed
  2. Zafra MP, et al. 2018. Nat Biotechnol. 36:888. PubMed
  3. Smeenk L, et al. 2021. Cancer Discov. 11:2868. PubMed
  4. Jang HK, et al. 2021. Sci Adv. 7:. PubMed
  5. Geisinger JM, et al. 2020. Nucleic Acids Res. 48:9067. PubMed
  6. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  7. Habib O, et al. 2022. Nucleic Acids Res. 50:1187. PubMed
  8. Siadous FA, et al. 2020. Autophagy. :1. PubMed
  9. Scheidmann MC, et al. 2022. Cancer Res. 82:681. PubMed
  10. Freitas GP, et al. 2021. Gene Ther. 28:748. PubMed
  11. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  12. Henriksson J, et al. 2019. Cell. 176:882. PubMed
RRID
AB_2749904 (BioLegend Cat. No. 844302)
AB_2565570 (BioLegend Cat. No. 844301)

Antigen Details

Structure
The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound.
Function
The Cas9 protein from S. pyogenes is part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
Biology Area
Cell Biology, Neuroscience
Antigen References

1. Mali P, et al. 2013. Science 339:823.

Gene ID
901176 View all products for this Gene ID
UniProt
View information about CRISPR on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08.05.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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