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Purified anti-Caspase-8 Antibody

Pricing & Availability
Clone
1H10 (See other available formats)
Regulatory Status
RUO
Other Names
MACH, FLICE, Mch5
Isotype
Rat IgG2a
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Product Citations
publications
1H10_PURE_Caspase-8_Antibody_1_WB_112817
Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using Caspase-8 (Clone 1H10, 1:1000 dilution, 0.5 µg/ml) rat primary antibody and HRP Goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1H10_PURE_Caspase-8_Antibody_1_WB_112817
    Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using Caspase-8 (Clone 1H10, 1:1000 dilution, 0.5 µg/ml) rat primary antibody and HRP Goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
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645501 25 µg 100€
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Description

Caspase-8 is involved in the apoptosis mediated by CD95 and TNFR-1. It is suggested that following the triggering of apoptosis by CD95 or TNFR-1, the adaptor molecule FADD interact physically with cytosolic caspase-8 and initiate the apoptotic cascade. The active caspase-8 consists of two subunits of p18 and p10. This antibody should recognize the whole length of human caspase-8 and some isofrms of lower MW.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length caspase-8 fusion protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
IHC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez I et al. 1999. Neuron. 22:623
  2. Li H, et al. 1998. Cell 94:491
Product Citations
  1. Huangfu J, et al. 2021. Cell Death Dis. 12:386. PubMed
  2. Khaiboullina S, et al. 2019. Front Pharmacol. 10:642. PubMed
RRID
AB_2068466 (BioLegend Cat. No. 645501)

Antigen Details

Structure
Full length protein of 55kd. Contains two homologous domains of DED (death effector domain) of FADD. The remainder of the protein is similar to the CED-3 subfamily of caspases.
Distribution

Expressed in multiple isoforms in multiple tissues. Relatively high level in peripheral leucocytes.

Function
Caspase-8 is involved in apoptosis mediated by CD95 and TNFR-1.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Muzio M et al. 1996. Cell 817-827
2. Cohen GM. 1997. Biochem J 326:1

Gene ID
841 View all products for this Gene ID
UniProt
View information about Caspase-8 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11.11.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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